本文件规定了基于DNA条形码技术在物种水平识别生态毒理学试验标本（主要是无脊椎动物和植物）的方案。执行DNA条形码的实验室可以使用该方案，以便尽可能标准化湿实验室和数据分析工作流程，并使其符合社区标准和指南。 本文件不打算为每种检测方法指定一种特定菌株，而是准确记录所使用的物种/菌株。 注1:这并不意味着DNA条形码化与每次测试运行并行进行，而是定期进行（例如，一年一次，如参比物质测试）以及每次开始新培养或向正在进行的培养中添加新个体时进行。 本文件无意复制或取代形态学-基于物种鉴定。相反，DNA条形码被提议作为形态学不确定的补充鉴定工具，或用于诊断隐性物种，以确保从不同生态毒理学实验室获得的结果指的是同一物种或菌株。 本文件适用于缺乏形态诊断特征（卵、幼虫、幼体）的未成熟形式的鉴定，以及现场监测研究中收集的标本的流线鉴定，其中对来自不同分类群的大量生物进行了分类。 注2原则上，生态毒理学测试中经常使用的所有物种都可以通过DNA条形码进行分析。除了地球上的赤子爱胜蚓（Eisenia fetida）和安德烈（E.andrei）之外，陆生物种的其他例子是地龙（Lumbricus terrestris）。rubellus、Allolobophora chlorotica、Aporrectodea rosea和A.caliginosa、Dendrodrilus rubidus、Enchytraeus albidus和E.crypticus(Haplotaxida)；念珠菌Folsomia candida、F.fimetaria、Proisotoma minuta和Sinella curviseta(弹尾目)；下丘脑、下丘脑、下丘脑（Acari）；双翅拟青虫(Aleochara bilineata)和铜翅拟青虫(Poecilus cupreus)(鞘翅目)；粪蝇、秋蝇(双翅目)或Pardosa sp.(蛛形纲)。线虫或蜗牛甚至植物也可以添加到这个列表中。

This document specifies a protocol to identify ecotoxicological test specimens (mainly invertebrates and plants) to the species level, based on the DNA barcoding technique. This protocol can be used by laboratories performing DNA barcoding in order to standardize both the wet-lab and data analysis workflows as much as possible, and make them compliant with community standards and guidelines. This document does not intend to specify one particular strain for each test method, but to accurately document the species/strain which was used. NOTE 1 This does not imply that DNA barcoding is performed in parallel to each test run, but rather regularly (e.g. once a year, such as reference substance testing) and each time a new culture is started or new individuals are added to an ongoing culture. This document does not aim at duplicating or replacing morphological-based species identifications. On the contrary, DNA barcoding is proposed as a complementary identification tool where morphology is inconclusive, or to diagnose cryptic species, in order to ensure that the results obtained from different ecotoxicological laboratories are referring to the same species or strain. This document is applicable to identifications of immature forms which lack morphological diagnostic characters (eggs, larvae, juveniles), as well as the streamline identification of specimens collected in field monitoring studies, where large numbers of organisms from diverse taxa are classified. NOTE 2 In principle, all species regularly used in ecotoxicological testing can be analysed by DNA barcoding. Besides the earthwoms Eisenia fetida and E. andrei, further examples for terrestrial species are Lumbricus terrestris, L. rubellus, Allolobophora chlorotica, Aporrectodea rosea, and A. caliginosa, Dendrodrilus rubidus, Enchytraeus albidus, and E. crypticus (Haplotaxida); Folsomia candida, F. fimetaria, Proisotoma minuta, and Sinella curviseta (Collembola); Hypoaspis aculeifer and Oppia nitens (Acari); Aleochara bilineata and Poecilus cupreus (Coleoptera); Scathophaga stercoraria, Musca autumnalis (Diptera) or Pardosa sp. (Arachnida). Nematodes or snails and even plants can also be added to this list.