1.1 这种环境室法测量了经过处理的蓝湿革和白湿革在四周内对孢子萌发和随后的营养生长的抵抗力。该测试方法有助于评估杀菌剂的性能，并有助于在真菌生长开始之前预测蓝湿革和白湿革的储存时间。该仪器的设计使任何相关方都可以轻松建造或获得，并复制蓝湿和白湿接种真菌孢子的自然环境。在未经处理或处理的蓝湿白上萌发的孢子可能会产生真菌生长，导致蓝湿白的变形或变色，或两者兼而有之。 1.2 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.3 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.4 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 6.1 环境箱法是一种加速试验，用于测定蓝湿革和白湿革对真菌（霉菌的病原体）生长的抗性。见测试方法 D3273 . 3. , 4. 6.2 环境室法有助于估计杀菌剂的性能，并有助于预测真菌生长开始前的储存时间。 6.3 环境室法复制了自然环境，在自然环境中，蓝湿革或白湿革接种了真菌孢子，随后被真菌破坏或变色。 6.4 环境室法测量处理后的蓝湿革或白湿革在四周内对孢子萌发和随后在相对较大的蓝湿革或白湿革样品表面蔓延的营养生长的抵抗力。 6.5 通过添加来自当前经营的制革厂的带有真菌生长的蓝湿革和白湿革样品，可以保持环境室接种代表制革厂中发现的真菌。 6.6 未经杀菌剂处理的蓝湿病和白湿病对照标本可以定期添加到培养箱中，以提高培养箱中真菌的生长水平。 6.7 在皮氏培养皿试验中，将杀真菌剂从试样中浸出到琼脂中通常会导致真菌生长抑制区，但在环境室中，从试样中浸出的任何杀真菌剂都会滴落到室内的水中，因此不会导致皮氏培养皿试验中观察到的错误读数。

1.1 This environmental chamber method measures the resistance of the treated Wet Blue and Wet White to the germination of spores and subsequent vegetative growth over a period of four weeks. The test method is useful in estimating the performance of fungicides and should assist in the prediction of storage time of Wet Blue and Wet White before fungal growth begins. The apparatus is designed so it can be easily built or obtained by any interested party and duplicate the natural environment in which Wet Blue and Wet White is inoculated with fungal spores. Spores that germinate on untreated or treated Wet Blue and Wet White can produce fungal growth, resulting in disfigurement or discoloration, or both, of the Wet Blue and Wet White. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 6.1 The environmental chamber method is an accelerated test for determining the resistance of Wet Blue and Wet White to the growth of fungi, the causal agent of mold. See Test Method D3273 . 3 , 4 6.2 The environmental chamber method is useful in estimating the performance of fungicides and should assist in the prediction of storage time before fungal growth begins. 6.3 The environmental chamber method duplicates the natural environment in which Wet Blue or Wet White is inoculated with fungal spores and subsequently disfigured or discolored by fungi. 6.4 The environmental chamber method measures the resistance of the treated Wet Blue or Wet White to the germination of spores and subsequent vegetative growth that spreads over the surface of a comparatively large Wet Blue or Wet White specimen over a period of four weeks. 6.5 The environmental chamber can be kept inoculated with fungi representative of those found in tanneries by adding samples of Wet Blue and Wet White with fungal growth from currently operating tanneries. 6.6 Control specimens of Wet Blue and Wet White without fungicide treatment can be added to the chamber periodically to increase levels of fungal growth in the chamber. 6.7 Leaching of fungicide from the test specimen into the agar often causes a zone of inhibition of fungal growth in the Petri dish test, but in the environmental chamber any leaching of fungicide from the test specimen drips into the water contained in the chamber and thus does not cause the types of false readings observed in the Petri dish test.