“CEN技术报告”的这一部分通过培养Salmonella spp的显色琼脂培养基上的个体菌落来确定定量复苏和计数的膜过滤程序。包括潜在的亚细菌损伤的沙门氏菌。在污水污泥中。它可能适用于其他污泥，土壤，土壤改良剂，生长介质和生物垃圾，但用户应使用这些材料验证方法。完全确定的范围将在提出的验证试验达成一致并进行后确定。 注1:目的是覆盖未经处理和处理的污泥，土壤，土壤改良剂，生长介质和生物量。 该方法特别适用于确定排污污泥中病原体的处理程序的效率，如“指令86/278/EEC（第3卷，CEN/TC 308-文件525”）所述。治疗A型过程最初通过使用诸如Salmonella senftenberg W775的测试生物体定义的Log10还原来验证。 该方法具有大约1 cfu/g湿重污泥的检测限，这取决于固体含量，高浓度（> 20％（w/v））可以限制样品体积通过膜的过滤，如果不是首先稀释。 注意

This part of the CEN Technical Report specifies a membrane filtration procedure for the quantitative resuscitation and enumeration, by culture of individual colonies on chromogenic agar media, of Salmonella spp. including potentially sub-lethally damaged Salmonella spp. in sewage sludges. It may be suitable for other sludges, soils, soil improvers, growing media and biowastes but the user shall validate the method using these materials. The fully defined scope will be determined after the proposed validation trials have been agreed and carried out. NOTE 1 The objective is to cover untreated and treated sludges, soils, soil improvers, growing media and biowastes. The method is particularly suited to determining the efficiency of treatment procedures for the elimination of pathogens in sewage sludge as outlined in the Revision of Directive 86/278/EEC (3rd Draft, CEN/TC 308 – doc 525). Treatment type A processes are initially to be validated through a to be defined Log10 reduction with a test organism such as Salmonella senftenberg W775. The method has a limit of detection of approximately 1 cfu/g wet weight sludge, dependent on the solids content which at high concentrations (> 20 % (w/v)) can restrict filtration of the sample volume through the membrane if not first diluted. NOTE 2 Salmonella spp. can be present in biosolids including untreated and treated sewage sludge as both vegetative and sub-lethally damaged cells; the latter require resuscitation to enable colony growth for accurate enumeration on agar media. NOTE 3 This method is not suitable for treated sludges containing less than 1 viable Salmonella spp. per 1 g wet weight. NOTE 4 This method is not suitable for untreated sludges containing low levels of Salmonella.