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Coliform Bacteria and Escherichia coli in Norwegian Drinking Water Sources - Comparison of Methods Based on the Fermentation of Lactose and Methods Based on the Activity of Specific Enzymes 挪威饮用水源中的大肠杆菌和大肠杆菌——基于乳糖发酵的方法与基于特定酶活性的方法的比较
发布日期: 2000-01-01
总大肠菌群(TC)、粪便大肠菌群(FC)和大肠杆菌是水微生物学中最常见的具有卫生意义的细菌指标。在挪威,地表水是饮用水的主要来源。约1800家供水厂为400万人口供水。当前的FC和TC分析挪威标准与国际标准化组织(ISO)给出的膜过滤和多管发酵方法非常相似。这两种方法都使用乳糖发酵来指示目标细菌的存在。然而,在过去三年中,挪威已经用基于酶的方法进行了初步研究。检查的方法基于确定的基质技术(Colilert-18/Quantitray(TM))。作为定量比较研究的介绍,通过乳糖和吲哚试验确认TC、FC和大肠杆菌与特定酶B的活性进行比较- D-半乳糖苷酶(GAD)和B-D-葡萄糖醛酸酶(GUD)。包括14个参考文献和表格。
Total coliform bacteria (TC), fecal coliform bacteria (FC), and Escherichiacoli are the most common bacterial indicators of sanitary significance in water microbiology. In Norway, surface waters are the main source of drinking water. Approximately 1800 water works supply a population of 4 million. The current Norwegian standard for FC and TC analyses are quite similar to the membrane filtration and multiple tube fermentation methods given by the International Standards Organization (ISO). Both methods use fermentationof lactose to indicate the presence of target bacteria. However, preliminary studies with enzyme-based methods have been carried out during the last three years in Norway. The method examined is based on defined substrate technology (Colilert-18/Quantitray(TM)). As an introduction to a quantitative comparative study, the confirmation of TC, FC, and E. coli by lactose and indole tests was compared with the activity of the specific enzymes B-D-galactosidase (GAD) and B-D-glucuronidase (GUD). Includes 14 references, tables.
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发布单位或类别: 美国-美国给水工程协会
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