1.1 本规程的目的是描述使用惯性冲击采样技术在琼脂平板上收集可培养的气载真菌孢子或碎片或细菌的程序。 1.2 本规程不包括通过不使用琼脂平板的设备收集可培养的真菌或细菌。 1.3 这种做法假设用户对与科学过程、抽样计划制定和实施相关的现场调查技术有基本了解。重要的是要建立待测试的相关假设和所需的支持分析方法，以确定要使用的采样介质和分析的实验室条件。 1.4 这种做法不涉及制定正式假设，也不涉及建立适当且有辩护理由的调查和抽样目标。据推测，研究人员具有解决这些问题的经验和知识库。 1.5 本规程未向用户提供足够的信息，以便解释样品采集的分析结果。用户有责任寻求或获得解释实验室报告的样本结果所需的信息和知识。 1.6 以国际单位制表示的数值应视为标准值。 本标准不包括其他计量单位。 1.7 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.8 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 4.1 本规程旨在使用惯性冲击法在琼脂平板上收集气载颗粒，以培养真菌或细菌。 4.2 当可培养气溶胶真菌和细菌的种类级鉴定或数量是室内空气质量调查的重要因素时，该做法很有价值。 4.3 用户有责任确保其遵守所有地方、州和联邦法规，这些法规管理建筑物的真菌和细菌定植检查以及相关样本的收集。 4.4 本规程旨在让用户基本了解使用惯性冲击器在琼脂平板上有效收集空气样本所需的设备、材料和说明。 4.5 本实践旨在最大限度地减少不同数据集之间的系统采样差异。

1.1 The purpose of this practice is to describe procedures for the collection of culturable airborne fungal spores or fragments or bacteria on agar plates using inertial impaction sampling techniques. 1.2 This practice does not include collection of culturable fungi or bacteria by devices not using agar plates. 1.3 This practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis. 1.4 This practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these issues. 1.5 This practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the user's responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory. 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 This practice is intended for the collection of airborne particles on agar plates using inertial impaction for the purpose of culturing fungi or bacteria. 4.2 This practice is valuable when species level identification or quantity of culturable aerosolized fungi and bacteria are important factors for the indoor air quality investigation. 4.3 It is the responsibility of the user to assure that they are in compliance with all local, state, and federal regulations governing the inspection of buildings for fungal and bacterial colonization and the collection of associated samples. 4.4 This practice is intended to provide the user with a basic understanding of the equipment, materials, and instructions necessary to effectively collect air samples on agar plates using an inertial impactor. 4.5 This practice is intended to minimize systematic sampling variations between different data sets.