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Benchmarking UV Collimated Beam Testing: Inter-laboratory Comparison of the UV Sensitivity of MS2 Coliphage 紫外线准直束测试的基准:MS2噬菌体紫外线敏感性的实验室间比较
发布日期: 2003-11-02
作为AWWARF项目2593的一部分,创新紫外线灭活病原体 科技公司(PI:James P.Malley教授)进行了“循环”紫外线(UV)消毒试验 在参与试验的实验室中进行,以估计试验中的不确定性水平 对MS2大肠杆菌噬菌体进行所谓的准直束紫外检测并完善方案 用于测定微生物的紫外线敏感性。 试验前进行了以下准备: 拟定了详细的实验方案,并由所有参与者进行审查; 所有辐射计都被同时送去进行校准(此外,还有一个辐射计) 被送到其他每个实验室直接比较读数 最后,使用KI/KIO3辐射计对所有辐射计进行了现场测试(Rahn,1997) 研究团队制定的方案);和 MS2库存和大肠杆菌宿主在一个实验室(MWD)中制备并运至 所有其他人都是通宵快递员。 进行了两次循环赛,一次在2001年3月,第二次在1月 2003年。这里展示了这些测试的结果,并对参与测试的人员进行了比较 实验室。此外,将结果与国家水研究所(NWRI)/美国水务协会研究基金会(AWWARF)设定的限制进行了比较。 MS2测试指南。 列表包括7个参考文献、表格、图表和附录。
As part of the AWWARF Project 2593, Inactivation of Pathogens by Innovative UV Technologies (PI: Professor James P. Malley), a "Round Robin" ultraviolet (UV) disinfection test was conducted among the participating laboratories to estimate the level of uncertainty in the performance of the so-called collimated beam UV test for MS2 coliphage and refine a protocol for determining the UV sensitivity of microorganisms. The following preparations were conducted prior to testing: a detailed Experimental Protocol was drafted out and vetted by all participants; all radiometers were sent to be calibrated at the same time (in addition, one radiometer was sent around to each of the other laboratories for direct comparison of readings, and finally, all radiometers were tested on site using the KI/KIO3 actinometer (Rahn, 1997) protocol developed by the research team); and, the MS2 stock and E. coli host were prepared in one laboratory (MWD) and shipped to all others by overnight courier. Two Round Robin series were conducted, one in March 2001 and the second in January 2003. The results of these tests are presented here and compared among the participating laboratories. In addition, the results are compared with the limits set by the National Water Research Institute (NWRI) / American Water Works Association Research Foundation (AWWARF) Guidelines for MS2 testing. list. Includes 7 references, tables, figures, appendix.
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发布单位或类别: 美国-美国给水工程协会
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