过去六年，佛罗里达州希尔斯堡县在其分布系统的西北部和中南部地区都检测到大肠菌群。此外，在过去两年中，总大肠菌群的检测率已超过样本率的5%。然而，这种现象表现出季节性，在夏末至初秋达到峰值，这与雨季和较低的用水量相吻合。该项目旨在研究配送系统内的微生物生态，以及任何公共卫生问题。分析包括使用分析剖面指数系统和Biolog对异养平板计数和大肠菌群进行物种化。 该项目还包括以米为单位分析从分配系统中移除的生物膜。仪表的选择基于其所在区域的服务记录，以及实现系统总体概述的基本原理。使用标准生化测试和聚合酶链反应对大肠菌群、致病菌（如分枝杆菌）和潜在病原体（如气单胞菌和假单胞菌）的存在与否进行调查。还调查了重要的生态细菌，如硝化菌群和硫酸盐还原菌群。数据显示，西北地区更容易受到个别大肠菌群物种进入系统的影响。 中南部地区似乎更受生物膜形成的影响，包括生物膜内硫酸盐还原菌的检测。在这两个地区都检测到嗜水气单胞菌。这些数据将与希尔斯堡县数据库中的数据相结合，以便为希尔斯堡县的客户提供更一致的水质。包括2个参考文献。

Hillsborough County, Florida, has detected coliform species within both the Northwest and South Central regions of its distribution system over the past six years. Furthermore, during the past two years, the detection of total coliforms has exceeded 5% of the sample prevelance. However, the occurrence showed a seasonality, peaking in late summer to early fall, which coincided with the rainy season and lower water consumption. This project was designed to study the microbial ecology within the distribution system, as well as any public health issues. Analysis included speciating heterotrophic plate count and coliform bacteria using the Analytical Profile Index system and Biolog. The project also included analysis of biofilms in meters removed from the distribution system. The meters were chosen based upon the service record of the area from which they were taken, as well as upon the rationale of achieving a general overview of the system. The presence or absence of coliforms, pathogenic bacteria, such as Mycobacterium, and potential pathogens like Aeromonas and Pseudomonas were investigated using standard biochemical tests and polymerase chain reaction. Ecologically important bacteria, such as nitrifying and sulfate-reducing groups, were also investigated. The data show that the Northwest region is more affected by individual coliform species entering the system. The South Central region seemed to be more affected by biofilm formation, including the detection of sulfate-reducing bacteria within biofilms. Aeromonas hydrophila was detected in both regions. The data will be combined with data from the Hillsborough County database in order to provide more consistent water quality to the customers of Hillsborough County. Includes 2 references.