饮用水系统的隐孢子虫污染仍然是一个公共卫生问题 用于饮用水行业。目前美国环境保护局（USEPA）检测人体隐孢子虫的方法 水（美国环保局方法1623）提供了卵囊存在或不存在的信息，但 没有提供关于分离物或卵囊是否具有传染性的信息。最近的一项研究使用 细胞培养结合聚合酶链反应（PCR）检测感染性卵囊的比例为1.4% 在从82个地表水处理厂采集的1690个经处理的饮用水样本中。像 作为这项早期研究的后续研究，3项检测将细胞培养与各种感染检测相结合 方法在两个实验室进行评估，以评估灵敏度、可靠性和准确性 （误报和漏报的频率）。建立了感染性检测方法 免疫荧光显微镜（IFA）、PCR和逆转录酶PCR（RT-PCR） 结合检测后基因分型。最可靠的分析将用于测试1000L 从参与的公用事业公司处采集处理过的饮用水样本，以检测是否存在传染性疾病 隐孢子虫卵囊。包括24个参考文献、表格和图表。

Cryptosporidium contamination of drinking water systems continues to be a public health issue for the drinking water industry. The current US Environmental Protection Agency (USEPA) methods for detecting Cryptosporidium in water (USEPA Method 1623) provides information on the presence or absence of oocysts, but gives no information on the isolate or whether the oocysts are infectious. A recent study using cell culture combined with polymerase chain reaction (PCR) detected infectious oocysts in 1.4% of 1,690 treated drinking water samples collected from 82 surface water treatment plants. As a follow-up to this earlier study, 3 assays combining cell culture with various infection detection methods were evaluated in 2 laboratories to assess sensitivity, reliability, and accuracy (frequency of false positives and false negatives). The infectivity detection methods were immunofluorescence microscopy (IFA), PCR, and reverse transcriptase-PCR (RT-PCR) combined with post-detection genotyping. The most reliable assay will be used to test 1000L treated drinking water samples from participating utilities for the presence of infectious Cryptosporidium oocysts. Includes 24 references, tables, figure.