1.1 本试验方法涵盖了一种标准化方法，用于定量测定抗菌化学品在处理被细菌孢子污染的坚硬、无孔表面时的有效性 C、 难辨的 （ATCC 43598）按照惯例生长 E2839年 . 1.2 本试验方法基于为试验方法制定的原则 E2197年 以及经济合作与发展组织的指导文件。 2. 1.3 进行该分析需要接受基本微生物学和无菌技术的培训。 1.4 单位- 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 该试验方法旨在测定在密闭系统中暴露于试验化学品后，坚硬无孔表面上孢子中的LR。 5.2 每个试验包括三个对照载体（暴露于含吐温的磷酸盐缓冲盐水中- 80），三个测试系统控制载体（暴露于1500 ppm ± 150 ppm次氯酸钠）和十种处理过的载体（每个测试化学品/浓度/接触时间组合）。

1.1 This test method covers a standardized approach to quantitatively determine the effectiveness of antimicrobial chemicals in treating hard, non-porous surfaces contaminated with spores of C. difficile (ATCC 43598) grown in accordance with Practice E2839 . 1.2 This test method is based on principles established for Test Method E2197 and an Organisation for Economic Co-operation and Development Guidance Document. 2 1.3 Training in basic microbiology and aseptic technique are required to perform this assay. 1.4 Units— The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 The test method was designed to determine the LR in spores on a hard, non-porous surface after exposure to a test chemical in a closed system. 5.2 Each test includes three control carriers (exposed to phosphate buffered saline with Tween-80), three test system control carriers (exposed to 1500 ppm  ± 150 ppm sodium hypochlorite), and ten treated carriers (per test chemical/concentration/contact time combination).