本文件规定了从陆地农田土壤中采样、处理和提取附着体的方法，作为将这些动物用作生物指示物的先决条件（例如，评估作为生物栖息地的土壤质量）。 参考书目中包含了关于飞虱生态及其在陆地环境中用作生物指示物的基本信息。 本文件适用于内生藻存在的所有陆地生物区。ISO 18400-101中给出了现场研究的总体抽样设计。参考文献[6]中给出了在现场条件下确定污染物对enchytraeids影响的概述，这些细节可能会因采样地点的气候/区域条件而异。 ISO 23611给出了蚯蚓或节肢动物等其他土壤生物群的测定方法- 1、ISO 23611-2、ISO 23611-4和ISO 23611-5。 本文件不适用于非常潮湿或淹水的土壤，在极端气候或地理条件下（如高山）可能难以使用。 在对土壤无脊椎动物进行采样时，强烈建议描述现场特征（例如，关于土壤性质、气候和土地利用）。然而，本文件不包括此类描述。ISO 10390、ISO 10694、ISO 11272、ISO 11274、ISO 11277、ISO 11461和ISO 11465更适合测量pH值、粒度分布、C/N比、有机碳含量和持水能力。

This document specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). Basic information on the ecology of enchytraeids and their use as bioindicators in the terrestrial environment is included in the Bibliography. This document applies to all terrestrial biotopes in which enchytraeids occur. The sampling design of field studies in general is given in ISO 18400-101. These details can vary according to the climatic/regional conditions of the site to be sampled and an overview on the determination of effects of pollutants on enchytraeids in field situations is given in Reference [6]. Methods for some other soil organism groups such as earthworms or arthropods are given in ISO 23611-1, ISO 23611-2, ISO 23611-4 and ISO 23611-5. This document is not applicable for very wet or flooded soils and might be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains). When sampling soil invertebrates, it is highly recommendable to characterize the site (e.g. concerning soil properties, climate and land use). However, such a characterization is not covered by this document. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 are more suitable for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity.