Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
动物饲料 取样和分析方法 微生物平板法筛选复合饲料中亚添加剂水平的泰乐菌素、维吉尼亚霉素、螺旋霉素、杆菌肽锌和阿伏帕辛
发布日期:
2017-06-21
BS EN 16936:2017提供了一种描述抗生素泰乐菌素筛选的方法,
完全喂养时亚添加剂水平的维吉尼亚霉素、螺旋霉素、杆菌肽锌和阿伏帕辛
通过微生物三平板试验检测原料和代乳品。该方法的检测限为1 mg/kg,用于阿沃帕霉素、泰乐菌素、螺旋霉素和维吉尼亚霉素,
杆菌肽锌为5mg/kg。其他(兽医)抗生素的存在可能会干扰治疗
方法此外,高浓度的金属(铜、锌)可能会产生干扰。该方法应被用作
定性筛选法。阳性结果可通过TLC进一步分析;确认
目的:需要LC-MS[1]。杆菌肽锌的检测下限(3 mg/kg)是可以达到的(见表2),但应
首先通过内部验证建立。交叉引用:EN ISO 13969(ISO 13969:2003)ISO 13969:2003第1版ISO 4833-1:2013第1版ISO 4833-1(ISO 4833-1:2013)ASEN ISO 4833-2(ISO 4833-2:2013)ASISO 4833-2:2013第1版ISO 7218:2007第3版EN ISO 7218(ISO 7218:2007)以及购买本文件时可用的所有现行修订。
BS EN 16936:2017 presents a method describing the screening on the antibiotics tylosin,
virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in complete feeding
stuffs and milk replacers by a microbiological 3-plate test.The limit of detection of the method is 1 mg/kg for avoparcin, tylosin, spiramycin and virginiamycin,
and 5 mg/kg for zinc bacitracin. The presence of other (veterinary) antibiotics may interfere with the
method.Furthermore, high concentrations of metals (Cu, Zn) may interfere. The method should be used as a
qualitative screening method. Positive results can be analysed further by TLC; for confirmatory
purposes LC-MS is required [1].A lower limit of detection for zinc bacitracin (3 mg/kg) is achievable (see Table 2), but should be
established with an in house validation first.Cross References:EN ISO 13969 (ISO 13969:2003)ISO 13969:2003 Ed 1 ISO 4833-1:2013 ED1EN ISO 4833-1 (ISO 4833-1:2013) ASEN ISO 4833-2 (ISO 4833-2:2013) ASISO 4833-2:2013 ED1ISO 7218:2007 Ed 3EN ISO 7218 (ISO 7218:2007) ASAll current amendments available at time of purchase are included with the purchase of this document.