可能被引入环境中的各种各样的威胁剂或污染物 国家的饮用水供应排除了对每种可能的药剂的检测。这迫使我们依赖 在一次试验中能够检测多种分析物的筛选方法。毒性试验 这是一种可能性。我们需要的是一个低成本、易于使用、可广泛部署的系统 Hach-ToxTrak法利用瑞苏林还原来测量呼吸，这是一种关键的呼吸 细胞存活途径。 瑞苏林是一种氧化还原活性染料，还原后会变色 从蓝色到粉色。对细菌有毒的物质会抑制它们的新陈代谢，从而 抑制瑞苏林减少的速度。这种对瑞苏林还原的抑制被视为 试验中的毒性指示。该方法使用促进剂（麸醛:美国。 专利5413916）中的方法，以减少反应时间，从而防止氧气干扰 同时，允许使用较低水平的接种物来减少染料。 使用 麸质醛允许在低成本仪器上进行测量，如分光光度计或 色度计。甚至使用色轮进行成本更低的视觉测量也被证明是有效的 工作可以使用任何类型的细菌培养。该方法已被评估并发现 能够有效检测饮用水中多种威胁因子的污染 包括重金属、除草剂、杀虫剂、灭鼠剂、放射性核素、工业化学品 包括氰化物、商业产品、生物毒素和化学战剂替代品。 这个 该方法被发现能有效地检测出几乎所有被测化合物的感兴趣水平 人类健康。该系统代表了一种成本效益高、适用范围广的哨兵方法，供用户使用 饮用水设施。该系统成本低，有效性广，易于使用 该系统非常适合发展以公民为基础的水监测组织，可以 用作发生污染事件时的预警系统。这种方法也可以 在第一反应人员和法医调查中寻找用于追踪污染物羽流或 验证系统入侵。包括7个参考文献、表格和图表。

The wide variety of possible threat agents or contaminants that could be introduced to the nation's drinking water supplies preclude testing for every possible agent. This forces us to rely on screening methods capable of detecting a diversity of analytes in a single test. Toxicity assays are one possibility. What is needed is a low cost easy to use system that can be widely deployed The Hach ToxTrak£ method makes use of resazurin reduction to measure respiration, a critical pathway for cell viability. Resazurin is a redox-active dye which, when reduced, changes color from blue to pink. Substances that are toxic to bacteria can inhibit their metabolism and thus inhibit the rate of resazurin reduction. This inhibition of resazurin reduction is taken as an indication of toxicity in the test. The method makes use of an accelerant (gluteraldehyde: U.S. patent 5,413,916) in the method to reduce the reaction time thus preventing oxygen interference and also, allowing the use of a lower level of inoculum to reduce the dye. The use of gluteraldehyde allows measurement on a low cost instrument such as a spectrophotometer or colorimeter. Even lower cost visual measurement with a color wheel has also been shown to work. Any type of bacterial culture may be used. This method has been evaluated and found to be effective in detecting contamination by a wide variety of threat agents in drinking water including heavy metals, herbicides, pesticides, rodenticides, radionuclides, industrial chemicals including cyanide, commercial products, biotoxins and chemical warfare agent surrogates. The method was found to be effective in detecting almost all compounds tested at levels of interest to human health. This system represents a cost effective and wide scope sentinel method for use by drinking water utilities. The low cost, wide range of effectiveness and ease of use entailed in this system make it ideal for the development of citizen based water monitoring groups that could be used as an early warning system in the event of a contamination incident. The method could also find use by first responders and in forensic investigations in tracing contaminant plumes or verifying system incursions. Includes 7 references, tables, figure.