Growing concerns over the presence of perchlorate in drinking water sources has promoted
research on treatment methods to remove this compound. Biological treatment using
packed bed reactors, such as biological activated carbon (BAC), has been proven to be a
viable option. Perchlorate is removed by perchlorate reducing bacteria (PRB) growing as
biofilms in BAC. PRB and other heterotrophs constitute the biofilm. PRB can utilize
perchlorate as electron acceptor. However, PRB can also use oxygen when available.
Therefore, maintaining low oxygen concentrations is important to accomplish removal of
perchlorate.
Backwashing in biological filters serves two purposes. First, regular backwashing is
necessary to prevent clogging and excessive pressure drops across the filter and, second,
backwashing is necessary to maintain an active biofilm and ensure good external mass
transfer of the contaminant to the biofilm (Hozalski and Bouwer, 1998). A previous study
has shown that biomass in BAC filters is retained both in the form of thin biofilm
attached to the surface of the carbon fibers and also in the form of large microbial
aggregates (up to several hundred micrometers) that accumulate in the inter-particle space
between the fibers (Choi et al., 2003). These large aggregates can be viewed as thick
biofilms that are diffusion limited in contrast to the thin biofilms on the carbon fibers that
are likely to be fully penetrated. Backwashing can be expected to washout a large fraction
of these loosely attached aggregates (Delahaye et al., 1999). Biofilm directly attached to the
carbon fibers is more resistant to shear and only a smaller fraction of this directly attached
biomass is expected to be lost during backwashing. Thus, backwashing should not only
reduce the total biomass in the system, but also is expected to change the relative amount of
thick biofilms (i.e., large microbial aggregates) to thin biofilms (directly attached to the
surface of the carbon fibers).
This paper demonstrates that large aggregates are important to maintain
perchlorate removal in cases of increased influent oxygen concentrations. Mass transfer
limitations in the large aggregates cause anaerobic zones to develop in the center of these
aggregates, allowing for perchlorate removal to take place. With increased oxygen
concentrations in the bulk phase, the thin biofilms on the surface of the carbon fibers will
be fully penetrated and will not allow for perchlorate removal to occur. Thus, the
hypothesis tested in this paper is that vigorous backwashing of a BAC filter has only a
limited influence on perchlorate removal as long as oxygen concentrations in the bulk
phase are low. However, after backwashing, BAC filters are more vulnerable to increased
bulk phase oxygen concentrations due to the loss of the majority of the large microbial
aggregates. To test this hypothesis, a lab-scale BAC filter was operated and perchlorate
removal was monitored before and after intensive backwashing.
Includes 6 references, figures.