这项研究的重点是利用“离片”技术对整个微小隐孢子虫卵囊的回收效率 提取技术。萃取方法分两个阶段进行评估:去除 覆盖层和安装介质；然后，将卵囊从载玻片转移到 基因分型管。用流式细胞术计数微小隐孢子虫卵囊中含有200、20或0 每孔卵囊数。然后取下并保留盖玻片，清洗水井。这个 叙述了被单和洗得很好的情况。结果表明，剩余卵囊的数量 在移除盖玻片和安装介质后，井上的压力变化很大，从 50%到95%。平均不到2%的卵囊附着在盖玻片上 剩余的卵囊用安装介质移除。这些结果表明，去除 coverslip对卵囊损失的解释很小；但是，必须使用保留在幻灯片上的安装介质 转移到基因分型管中，以准确反映载玻片上的卵囊。包括8篇参考文献，仅限于摘要。

This study focuses on recovery efficiencies of whole Cryptosporidium parvum oocysts using "off-the-slide" extraction techniques. The extraction approach was evaluated in two phases: removal of the coverslip and mounting media; and, transfer of the oocysts from the slide to the genotyping tube. C. parvum oocysts were counted by flow cytometry to contain 200, 20 or 0 oocysts per well. The coverslip was then removed and retained, and the well was washed. The coverslip and washed well were recounted. Results show that the number of oocysts remaining on the well was variable after removal of the coverslip and mounting media, ranging from nearly 50% to 95%. Less than 2% of the oocysts on average were attached to the coverslip, with the remaining oocysts removed with the mounting media. These results suggest that removal of the coverslip accounts for little oocyst loss; however, the mounting media retained on the slide must be transferred to the genotyping tube to accurately reflect the oocysts on the slide. Includes 8 references, abstract only.