1.1 这些测试方法包括通过使用细菌、酵母或真菌培养物挑战粘附样本，并检查其恢复无菌的能力，来测定液体粘附制剂对容器中微生物攻击的耐受性。这些测试方法返回定性结果。 1.2 以国际单位制表示的数值应视为标准。括号中的值仅供参考。 1.3 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 这些测试方法旨在供受过正确微生物技术培训的人员使用。第节给出了具体的预防说明 8. . 1.4 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 这些测试方法用于证明粘合剂制剂在其储存期内是否受到杀生物剂的充分保护，以抵抗细菌、酵母和真菌的攻击。它们是按照生物实验室用于粘合剂行业的方法制作的。 5.2 这些测试方法也可用于确定不同杀生物剂系统对特定微生物的功效。 5.3 当针对从受污染的粘合剂中分离出来的野生型微生物进行测试时，这些测试方法特别有用，以帮助确定杀死或抑制污染物生长所需的杀生物剂的数量和类型。如果选择一种通常不用作挑战性生物体的分离微生物作为接种物，则重要的是识别该生物体，并确定其在何种培养基上生长以及在何种条件下生长，以证明杀生物剂的功效。 5.4 使用这些方法中给出的程序时获得的结果仅适用于用于测试的物种。 第节中列出的测试物种 9 它们经常被实验室用来测试抗菌性能，但它们不是唯一可以使用的。选择用于这些测试方法的物种需要测试实验室或要求测试的一方进行知情判断。使用通常会侵蚀粘合剂的品种也很重要。看见 9.4 . 5.5 从粘附样本携带到琼脂的活性杀生物剂的存在可能会对微生物的生长产生抑制作用，导致在正常潜伏期内没有生长，而事实上，存在活的微生物，但它们的生长已经放缓或停滞。 建议使用Letheen琼脂和肉汤来中和这种遗留物的影响。 注4: Letheen琼脂可用于划线板，或者如果选择另一种琼脂进行测试，则可以划线Letheen琼脂板作为对照，以测试中和效果。更有效的方法是用Letheen肉汤稀释受挑战的粘附样本，并运行Letheen琼脂倾注板。看见 注1 和 附注3 . 延长负极的潜伏期将是另一项保障措施。为了中和基于噻唑啉的防腐剂，可以向培养基中添加10至50 ppm的硫代乙醇酸钠。 5.6 这些测试方法取决于所报告的一组条件下活微生物的生理作用。 通过将结果与已知耐药性的同时运行对照进行比较，可以得出关于测试粘合剂抗微生物侵蚀的结论。看见 X5.2 关于测试重复性的声明。

1.1 These test methods cover the determination of the resistance of liquid adhesive preparations to microbial attack in the container by challenging adhesive specimens with cultures of bacteria, yeast, or fungi, and checking for their ability to return to sterility. These test methods return qualitative results. 1.2 The values stated in SI units are to be regarded as the standard. The values in parentheses are for information only. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological technique. Specific precautionary statements are given in Section 8 . 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 These test methods are used to demonstrate whether an adhesive preparation is sufficiently protected with biocide to resist attack by bacteria, yeast, and fungi during its storage life. They are patterned after methods used by biological laboratories serving the adhesive industry. 5.2 These test methods may also be used to determine the efficacy of different biocide systems against specific microorganisms. 5.3 These test methods are especially useful when tested against wild-type microorganisms which have been isolated from contaminated adhesives as an aid in determining the amount and type of biocide necessary to kill or inhibit the growth of the contaminants. If an isolated microorganism not generally used as a challenge organism, is chosen as the inoculum, it is important to identify the organism and determine on which medium and under what conditions it will grow, in order to demonstrate the efficacy of the biocide. 5.4 The results obtained when using the procedures given in these methods apply only to the species which are used for the testing. The test species listed in Section 9 are frequently used by laboratories to test for antimicrobial properties, but they are not the only ones which could be used. Selection of the species to use for these test methods requires informed judgment by the testing laboratory or by the party requesting the tests. It is also important that species which commonly attack adhesives be used. See 9.4 . 5.5 The presence of an active biocide carried over from the adhesive specimen to the agar could have an inhibiting effect on the growth of microorganisms, resulting in no growth during the span of a normal incubation period, when in fact, viable microorganisms are present, but their growth has been slowed down or held in stasis. The use of Letheen agar and broth is recommended to neutralize the effect of this carry-over. Note 4: Letheen agar may be used for the streak plates, or if another agar is chosen for testing, a Letheen agar plate could be streaked as a control to test against the neutralizing effect. Even more effective would be diluting the challenged adhesive specimen with Letheen broth and running Letheen agar pour plates. See Note 1 and Note 3 . Extending the incubation period of negative plates would be another safeguard. To neutralize thiazoline-based preservatives, 10 to 50 ppm of sodium thioglycolate can be added to the medium. 5.6 These test methods are dependent upon the physiological action of living microorganisms under a reported set of conditions. Conclusions about the resistance of the test adhesive to microbiological attack can be drawn by comparing the results to simultaneously run controls of known resistance. See X5.2 for statements regarding test repeatability.