1.1 本试验方法包括测定硬木材和软木材、草本材料（如柳枝稷和蚕桑）、农业残留物（如玉米秸秆、小麦秸秆和甘蔗渣）、废纸（如办公垃圾、纸板和新闻纸）、酸和碱预处理的生物质的酸不溶性残留物，以及发酵残留物的固体部分。所有结果都是相对于105 °C烘箱干燥的样品重量。 1.2 所收集的残留物含有酸不溶性木质素和来自原始样品的任何浓缩蛋白质。需要进行独立的氮分析，以确定与浓缩蛋白质组分分离的酸不溶性木质素含量，这不在本试验方法的范围内。 1.3 在该过程中，一些生物质样品中的一部分木质素将保持可溶。生物质样品中的总木质素包括酸溶性木质素和酸不溶性残留物中的木质素。 1.4 以国际单位制表示的数值应视为标准。本标准不包括其他计量单位。 1.5 本标准并不旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 具体危害说明见第节 8. 和 注3 和 注5 . 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 4.1 酸不溶性残留物含量与其他测定法一起用于测定生物质样品的总组成。

1.1 This test method covers determination of the acid-insoluble residue of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105 °C oven-dried weight of the sample. 1.2 The residue collected contains the acid-insoluble lignin and any condensed proteins from the original sample. An independent nitrogen analysis would be required to determine the acid-insoluble lignin content separate from the condensed protein fraction and is outside the scope of this test method. 1.3 A portion of the lignin in some biomass samples will remain soluble during this procedure. The total lignin in a biomass sample includes both acid-soluble lignin and lignin in the acid insoluble residue. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8 and Note 3 and Note 5 . 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 The acid-insoluble residue content is used in conjunction with other assays to determine the total composition of biomass samples.