该欧洲标准规定了高效液相色谱（HPLC）方法，用于确定动物饲料中授权水平的半纤维素含量[2]，使用质谱检测或柱后衍生化和（UV）-VIS检测（下文UV检测）。这种方法适用于家禽饲料。当使用质谱法进行检测时，定量限为1.0 mg/kg，当采用柱后衍生法进行紫外检测时，定量限为3.0 mg/kg。定量下限是可以实现的，但这是由用户验证的。 该方法允许鉴别来自莫能菌素，盐霉素，假丝氨酸，马杜拉霉素和拉沙里菌素的半纤维素

This European standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the semduramicin content at authorized level in animal feeding stuffs [2], using mass spectrometry detection or post-column derivatization and (UV)-VIS detection (hereinafter UV detection). This method is applicable to poultry feed. The limit of quantitation is 1,0 mg/kg when mass spectrometry is used for detection and 3,0 mg/kg when the detection is performed by UV with post-column derivatization. Lower limits of quantitation are achievable but this is to be validated by the user. The method allows the discrimination of semduramicin from monensin, salinomycin, narasin, maduramicin and lasalocid.