越来越多的荧光染色剂和探针可用于在单个细胞水平上对细菌生理学的各个方面进行原位细胞内评估。在这项研究中，将一套这些染色剂和探针与活菌平板计数结合使用，以评估氯消毒对膜电位[Rh123和DiBAC4（3）]、膜完整性（活/死BacLight试剂盒）、呼吸活性（CTC）、微菌落形成（MicroStar J系统）、酯酶活性（Scanderi J系统）的影响，共生病原体E.coli O157:H7中的底物反应性（DVC）和消毒抗性。饥饿14天后，DVC值略有下降，而所有其他检测结果保持相对稳定和等效。在饥饿期间，抗氯性逐渐增强。到29天时，对照培养物和用氯气处理的培养物之间没有显著差异（0。 5毫克/升）。如本研究所示，通过检测多个目标对生理活性进行原位评估，可以更全面地确定亚致死消毒后细菌细胞损伤的部位和程度。这些原位技术通过提供有关细菌生理变化的信息来评估氯诱导的损伤，并支持其在快速检测应激细菌方法中的应用。

A growing number of fluorescent stains and probes are available that allow in situ, intracellular assessment of various aspects of bacterial physiology at the level of the individual cell. In this study, a suite of these stains and probes were used in conjunction with viable plate counts to assess the effect of chlorine disinfection on membrane potential [Rh123 and DiBAC4(3)], membrane integrity (LIVE/DEAD BacLight kit), respiratory activity (CTC), microcolony formation (MicroStar J system), esterase activity (ScanRDI J system), substrate responsiveness (DVC) and disinfection resistance in the commensal pathogen E.coli O157:H7. After 14 days of starvation, DVC values slightly decreased while all other assays remained relatively constant and equivalent. Chlorine resistance progressively increased through the starvation period. By 29 days, there was no significant difference between control cultures and those that had been treated with chlorine (0.5 mg/L). In situ assessment of physiological activity by examining multiple targets, as demonstrated in this study, permits a more comprehensive determination of the site and extent of injury in bacterial cells following sublethal disinfection. These in situ techniques allow the assessment of chlorine induced injury by providing information on altered bacterial physiology and support their application in rapid methods for detecting stressed bacteria.