用于检测大肠杆菌O157:H7的传统方法很费力 而且很费时。现有的方法缺乏检测低水平辐射的灵敏度 未经处理的原水或成品自来水中可能存在的污染。 这篇手稿描述了该技术的发展和多实验室验证 使用免疫学或分子检测来检测 >一天内每毫升大肠杆菌O157:H7 0.05 CFU。这些方法可以 方便地衔接到总大肠菌群分析的现有程序中，或作为 独立程序。使用这些程序将允许公用事业公司 积累重要的流行病学信息，并采取应急措施 保护公众健康免受这种重要的人类病原体的侵害。包括12个参考文献和表格。

The conventional methods employed for detection of E. coli O157:H7 are laborious and time consuming. Existing methods lack the sensitivity to detect low levels of contamination that would be anticipated in untreated raw or finished tap water. This manuscript describes the development and multi-laboratory validation of procedures that employ immunological or molecular detection to enable detection of >0.05 CFU per mL of E. coli O157:H7 within a single day. The methods can conveniently dovetail into existing procedures for total coliform analyses or serve as stand-alone procedures. Employment of these procedures will allow utilities to accrue important epidemiological information and employ contingency measures to safeguard public health from this significant human pathogen. Includes 12 references, tables.