本文件是EN 13130的一部分，规定了在食品模拟物水，3％w/v乙酸水溶液中测定3,3-双（3-甲基-4-羟基苯基）-2-二氢吲哚酮的分析程序，15 ％v/v含水乙醇和橄榄油或批准的替代品。的水平 测定的3,3-双（3-甲基-4-羟基苯基）-2-二氢吲哚酮的摩尔数表示为毫克数 3,3-双（3-甲基-4-羟基苯基）-2-二氢吲哚酮/千克食品或食品模拟物。该方法适用于在近似分析物浓度范围为0.18mg/kg至4/kg的食品模拟物中定量测定3,3-双（3-甲基-4-羟基苯基）-2-二氢吲哚酮。 注1:该方法还应适用于其他水性食品模拟物以及其他脂肪食品模拟物。合成甘油三酯或向日葵油的混合物。 注2:在设置迁移试验之前，应对脂肪模拟物的适用性进行评估。发现橄榄油在某些情况下给予不可接受的干扰

This document, part of EN 13130, specifies an analytical procedure for the determination of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indolinone in the food simulants water, 3 % w/v aqueous acetic acid, 15 % v/v aqueous ethanol and olive oil or approved substitute. The level of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indolinone determined, is expressed as milligrams of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indolinone per kilogram of food or food simulant. The method is applicable to the quantitative determination of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indolinone in an approximate analyte concentration range of 0,18 mg/kg to 4/ kg of food simulants. NOTE 1 The method should also be applicable to other aqueous food simulants as well as to the other fatty food simulants e.g. a mixture of synthetic triglycerides or sunflower oil. NOTE 2 The suitability of the fat simulant should be assessed prior to setting up migration tests. Olive oil has been found to give unacceptable interference in some cases.