BS EN ISO 17601:2018规定了定量实时聚合酶链的关键步骤 反应（qPCR）法测量土壤DNA中选定微生物基因序列的丰度 提供选定微生物群估计值的提取物。值得注意的是，基因的数量不一定与生物体的数量直接相关 这些都是经过测量的。例如，核糖体操纵子的数量从一个拷贝到20个拷贝不等 在不同的细菌门中。因此，从土壤DNA中量化的16S rRNA序列的数量 提取物不能准确估计土壤细菌的数量。此外 序列不一定与活的微生物有关，可以包含扩增的序列 来自死亡微生物。交叉引用:ISO 11063ISO 10381-6包含以下内容:勘误表，2018年3月

BS EN ISO 17601:2018 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.Cross References:ISO 11063ISO 10381-6Incorporates the following:Corrigendum, March 2018