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Recovery of Cryptosporidium From Water Samples Using the ProNetic(TM) System 用ProNetic(TM)系统从水样中回收隐孢子虫
发布日期: 1998-01-01
一种新的隐孢子虫检测系统——ProNetic(TM)系统已经开发出来,它利用免疫磁性分离(IMS)从水样中纯化卵囊。生物体的可视化和计数是通过免疫荧光染色和使用荧光显微镜进行观察来实现的。仅使用ProNetic(TM)IMS系统,并结合Gelman Envirochek(TM)过滤,检查从加标样本中回收卵囊的情况。结果表明,与其他使用《信息收集规则》(ICR)中概述的美国环境保护局方法报告的结果相比,ProNetic(TM)系统提供了更好的回收率和再现性。 使用Envirocheck(TM)过滤器和ProNetic(TM)系统,分析员可以在大约四小时内检测到10升水样中是否存在隐孢子虫。
A new Cryptosporidium detection system, the ProNetic(TM) System, has been developed which utilizes immunomagnetic separation (IMS) to purify oocysts from water samples. Visualization and enumeration of organisms is achieved by immunofluorescent staining and observation using an epifluorescence microscope. Recovery of oocysts from spiked samples was examined using the ProNetic(TM) IMS System alone, and in combination with Gelman Envirochek(TM) filtration. The results indicate that the ProNetic(TM) System provides improved recovery and reproducibility when compared to results previously reported by others using the USEPA method outlined in the Information Collection Rule (ICR). Using the Envirocheck(TM) filter in combination with the ProNetic(TM) System, allowed the analyst to detect the presence of Cryptosporidium in a 10 liter water sample in approximately four hours.
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发布单位或类别: 美国-美国给水工程协会
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