BS EN 17049:2018规定了高效液相色谱法？用于鉴定动物饲料中泰乐菌素、螺旋霉素、维吉尼亚霉素、卡巴多和喹乙醇的质谱（LC-MS/MS）方法。本方法适用于复合动物饲料中低浓度泰乐菌素、螺旋霉素、维吉尼亚霉素、卡巴多和喹乙醇的鉴别。泰乐菌素、螺旋霉素和维吉尼亚霉素的鉴别限度为1 mg/kg，卡巴多的鉴别限度为4 mg/kg，喹乙醇的鉴别限度为3 mg/kg。该方法在一项合作研究中得到了充分验证（见附件a）。由于泰乐菌素、螺旋霉素和维吉尼亚霉素是由几种密切相关的化合物混合而成的发酵产物，因此分析基于对最丰富成分的检测和鉴定。泰乐菌素的标记物是泰乐菌素A，螺旋霉素的标记物是螺旋霉素I和II，维吉尼亚霉素的标记物是维吉尼亚霉素M1和S1。 其他异构体和形式可以用相同的方法很容易检测到，但需要根据前体和产物离子的分子质量调整MS参数。对卡巴多和喹乙醇进行了分析。交叉引用:EN ISO 6498ISO 6498EN ISO 6497ISO 6497购买本文件时提供的所有当前修订版均包含在购买本文件中。

BS EN 17049:2018 specifies a high performance liquid chromatography?mass spectrometry (LC-MS/MS) method for the identification of tylosin, spiramycin, virginiamycin, carbadox and olaquindox in animal feeds.The method is suitable for the identification of low concentrations of tylosin, spiramycin, virginiamycin, carbadox and olaquindox in compound animal feeds. A limit of identification of 1 mg/kg for tylosin, spiramycin and virginiamycin, 4 mg/kg for carbadox and 3 mg/kg for olaquindox should be obtained by using the described method. The method was fully validated during a collaborative study (see Annex A).Since tylosin, spiramycin and virginiamycin are fermentation products consisting of a mixture of several closely related compounds, the analysis is based on detection and identification of the most abundant constituents. For tylosin the marker is tylosin A, for spiramycin the marker is spiramycin I and II and for virginiamycin the marker is virginiamycin M1 and S1. The other isomers and forms can be readily detected with the same method but adjustment of the MS parameters according to the molecular mass of precursor and product ions need to be made. Carbadox and olaquindox are analysed as such.Cross References:EN ISO 6498ISO 6498EN ISO 6497ISO 6497All current amendments available at time of purchase are included with the purchase of this document.