Water quality — Detection and enumeration of bacteriophages — Part 4: Enumeration of bacteriophages infecting Bacteroides fragilis
水质与灰分;噬菌体SPART 4的检测和计数:感染脆弱类杆菌的噬菌体计数
发布日期:
2001-12-20
ISO 10705的这一部分规定了检测和计数感染拟杆菌的噬菌体的方法
通过将样品与适当的宿主菌株孵育来培养脆弱菌株。该方法适用于各种水,
沉积物和污泥提取物,必要时稀释后。在低噬菌体数量的情况下,预浓缩
步骤可能是必要的,为此已经制定了单独的国际标准。方法
也适用于贝类提取物。
注意:希望尽可能广泛地采用国际标准。ISO 10705的这一部分包括参考
不需要昂贵的材料或设备的替代程序
发展中国家。使用这些替代方案不会影响该方法的性能。
This part of ISO 10705 specifies a method for the detection and enumeration of bacteriophages infecting Bacteroidesfragilis by incubating the sample with an appropriate host-strain. The method is applicable to all kinds of water,
sediments and sludge extracts, where necessary after dilution. In the case of low phage numbers, a preconcentration
step may be necessary for which a separate International Standard has been developed. The method
is also applicable to shellfish extracts.
NOTE It is desirable for International Standards to be adopted as widely as possible. This part of ISO 10705 includes reference
to alternative procedures which obviate the need for expensive materials or equipment which may not be readily available in
developing countries. Use of these alternatives will not affect the performance of this method.