ISO 17512-1:20 08规定了一种快速筛选方法，用于评估土壤的栖息地功能以及污染物和化学物质对蚯蚓行为的影响。 亚致死试验是一种快速方法，反映了天然土壤中污染物混合物和掺入土壤中的物质对赤子爱胜蚓和安德烈爱胜蚓的生物利用度。蠕虫的回避行为是测试的测量终点。该测试不打算取代蚯蚓繁殖测试。 已经开发并成功应用了两种不同的设计（两段单元和六段单元）。两种设计都适用于单浓度（例如用于评估田间土壤的质量）或多浓度（例如用于评估加标化学品的毒性）测试。在这两种情况下，允许蚯蚓对哪个隔室、对照和处理进行初始选择【在右侧和左侧之间的两段测试容器中；在（3+3）交替隔室之间的六段测试容器中】，进入。

ISO 17512-1:2008 specifies a rapid screening method for evaluating the habitat function of soils and the influence of contaminants and chemicals on earthworm behaviour. The sublethal test is a rapid method that reflects the bioavailability of contaminant mixtures in natural soils and substances spiked into soils to Eisenia fetida and Eisenia andrei. The avoidance behaviour of the worms is the measurement endpoint of the test. This test is not intended to replace the earthworm reproduction test. Two different designs (a two section unit and a six section unit) have been developed and successfully applied. Both designs are applicable to either single-concentration (e. g. for assessing the quality of a field soil) or multi-concentration (e. g. for assessing the toxicity of a spiked chemical) tests. In both cases, the earthworms are allowed to make the initial choice on which compartment, control and a treatment [in the two section test vessel between right and left side; in the six section test vessel between the (3 + 3) alternating compartments], to enter.