This slide presentation outlines information on regulatory testing of pathogen reduction by microbial water treatment devices. Topics covered include: U.S. Environmental Protection Agency (USEPA) Purifier Guide Standard, protocol and test conditions; challenge cycles; general test water; plumbed system set-up; microbial challenge; detection methods for microbials; protozoan viability; Cryptosporidium in neonatal mouse and in cell culture; Giardia infectivity (viability); challenge points; measure of purifier; and, bacteriophage.