ISO/TS 17764-1:20 02规定了两种制备复合动物饲料原料的动物和植物脂肪、油和脂肪酸混合物的脂肪酸甲酯的方法，以及源自动物饲料的脂肪提取物的脂肪酸，包括含有丁酸的脂肪和脂肪酸混合物。 一般方法三氟化硼(BF3)方法涉及制备源自脂肪、油和游离脂肪酸的具有六个或更多个C原子的脂肪酸甲酯。 KOH/HCl法涉及具有四个或更多个C原子的脂肪酸甲酯的制备。该方法也可用于游离脂肪酸混合物中链长小于10个C原子的脂肪酸的定量测定。 生产的甲酯可用于气体-液相色谱(GLC)。 不皂化物质不被除去，并且当以相当大的量存在时，会干扰色谱分析。 ISO/TS 17764-2:20 02描述了通过使用根据ISO/TS 17764-1:20 02中规定的方法获得的脂肪酸甲酯，使用具有毛细管柱的气相色谱法和火焰离子化检测来测定脂肪中脂肪酸的含量。

ISO/TS 17764-1:2002 specifies two methods for preparing the methyl esters of fatty acids of animal and vegetable fats, oils and fatty acid mixtures for raw materials for compound animal feeds, and fatty acids originating from fat extracts of animal feeding stuffs, including fats and fatty acid mixtures containing butyric acid. The general method, the boron trifluoride (BF₃) method is concerned with the preparation of methyl esters of fatty acids with six or more C atoms, originating from fats, oils and free fatty acids. The KOH/HCl method is concerned with the preparation of methyl esters of fatty acids with four or more C atoms. This method can also be used for the quantitative determination of fatty acids with a chain length shorter than 10 C atoms in free fatty acid mixtures. The methyl esters produced can be used for gas-liquid chromatography (GLC). Unsaponifiable materials are not removed and can, when present in considerable amounts, interfere with the chromatographic analysis. ISO/TS 17764-2:2002 describes the application of gas chromatography with capillary columns and flame ionization detection for the determination of the content of fatty acids in a fat by making use of the methyl esters of the fatty acids obtained in accordance with the methods specified in ISO/TS 17764-1:2002.