The performance of two laboratory-scale hollow fiber membrane bioreactors (HFMBs)was investigated for biological denitrification of nitrate (NO3-) contaminateddrinking water. Both a hydrogenotrophic HFMB and a heterotrophic HFMB were used.In the hydrogenotrophic HFMB, H2 flows through the lumen of the hydrophobicmicroporous hollow fibers and diffuses to the attached biofilm. Nitratecontaminated water circulates on the shell side of the reactor. H2 is utilized bythe hydrogenotrophic population creating a driving force for mass transfer.Advantages of the hydrogenotrophic HFMB include high gas transfer rates, highbiomass densities and bubbless operation. In the heterotrophic HFMB, NO3-contaminated water flows through the lumen of the microporous hollow fibers andNO3- diffuses through the pores of the membrane. NO3- is utilized by theheterotrophic population circulating on the shell side of the reactor, creating adriving force for mass transfer. The microporous membranes provide a high nitratepermeability, while separating the microbial population from the water beingtreated. This eliminates the possibility of carryover of microbial biomass intothe product water and allows for independent optimization of each phase of thesystem. Both reactors achieved high (>97%) NO3- removal efficiencies even at veryhigh influent NO3- concentrations. Water quality testing showed a significantincrease in turbidity, total organic carbon (TOC) and dissolved organic carbon(DOC) in the hydrogenotrophic HFMB, possibly due to leaking microbial productsand sloughed biomass. In the heterotrophic HFMB, approximately 8% of the addedmethanol partitioned into the product water through the membranes. Includes 36 references, table, figures.