该欧洲标准定义了通过质谱检测（MS）分析饲料中1mg/kg至100mg/kg浓度范围内的三聚氰胺和氰尿酸的高效液相色谱法（HPLC）。该方法适用于筛选和确认（定量）目的。已经证明该方法的定量限度优于三聚氰胺的0.3mg/kg和氰尿酸的0.6mg/kg。浓度超过100mg/kg的量化是可能的，但该方法必须由操作者验证

This document specifies a high-performance liquid chromatographic (HPLC) mass spectrometric (MS) method for screening and quantification of melamine and cyanuric acid in the concentration range between 1 mg/kg and 100 mg/kg feed. The method is validated in an international collaborative trial for melamine in complete feed, complementary feed, feed material, milk replacer and pet food including canned pet food in the range between 1 mg/kg and 80 mg/kg with particular regard to the maximum level of 2,5 mg/kg as established by the European Commission. Laboratory experiences have shown that the method is also applicable for cyanuric acid in the same concentration range in complete feed (n = 7), complementary feed (n = 6), feed material (n = 7, resp. 9), milk replacer (n = 7) and pet food (n = 7) including canned pet food. Since the LC-MS/MS sensitivity for cyanuric acid is lower than for melamine, it has to be ensured that the LC-MS/MS system is in excellent working order. The method is applicable to feeding stuffs but not tested for pre-mixtures and feed additives. Quantification of concentrations above 100 mg/kg is possible, but the method has to be validated by the operator.