This paper presents energy and life cycle climate performance (LCCP) analyses of a variety of supermarket refrigeration systems to identify designs that exhibit low environmental impact and high energy efficiency. EnergyPlus was used to model refrigeration systems in a variety of climate zones across the United States. The refrigeration systems that were modeled include the traditional multiplex DX system, cascade systems with secondary loops and the transcritical CO2system. Furthermore, a variety of refrigerants were investigated, including R-32, R-134a, R-404A, R-1234yf, R-717, and R-744. LCCP analysis was used to determine the direct and indirect carbon dioxide emissions resulting from the operation of the various refrigeration systems over their lifetimes. Our analysis revealed that high-efficiency supermarket refrigeration systems may result in up to 44% less energy consumption and 78% reduced carbon dioxide emissions compared to the baseline multiplex DX system. This is an encouraging result for legislators, policy makers and supermarket owners to select low emission, high-efficiency commercial refrigeration system designs for future retrofit and new projects.