本文件规定了使用反相高效液相色谱-串联质谱（LC-MS/MS）对烟草和以下烟草产品中的四种烟草特有亚硝胺（TSNA）进行定量的方法:香烟、雪茄和无烟烟草产品。用该方法测定的TSNA为:N-亚硝基鸟嘌呤（NNN）、N-亚硝基鸟嘌呤（NAT）、N-亚硝基鸟嘌呤（NAB）和4-（甲基亚硝胺）-1-（3-吡啶基）-1-丁酮（NNK）。

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in tobacco and the following tobacco products: cigarettes, cigars and smokeless tobacco products using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The TSNAs determined with this method are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).