Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 1: Detection of Enterobacteriaceae
食物链的微生物学 - 肠杆菌科检测和计数的水平方法 - 第1部分:肠杆菌科检测
发布日期:
2017-06-16
ISO 21528-1:20 17规定了一种用于检测肠杆菌科细菌的富集方法。它适用于
-拟供人食用及喂养动物的产品,及
-初级生产、食品生产和食品处理领域的环境样本。
此方法适用
-当预期所寻找的微生物需要通过富集复苏时,以及
-当所寻求的数量预期低于每毫升或每克供试品100个时。
ISO 21528-1:20 17的适用性受到该方法对很大程度可变性的敏感性的限制(参见第11条)。
注:计数可以通过在液体培养基中孵育后计算最可能数(MPN)来进行。见附件A。
ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This method is applicable
- when the microorganisms sought are expected to need resuscitation by enrichment, and
- when the number sought is expected to be below 100 per millilitre or per gram of test sample.
A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).
NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.