在感染性试验中，人类宫颈癌（HCT-8）细胞被不同浓度的微小隐孢子虫卵囊感染。孵育48小时后，使用地高辛标记的DNA探针对微小隐球菌特异性小亚单位rRNA序列进行原位杂交（ISH），并进行比色检测，以检测感染性卵囊。此外，使用荧光素标记的抗微小隐球菌子孢子的多克隆抗体对微小隐球菌的细胞内繁殖阶段进行了免疫荧光检测，并与ISH进行了比较。小隐孢子虫和小鼠隐孢子虫都被用来感染HCT-8细胞和ISH，以确定DNA探针的特异性。包括18个参考文献、表格、图表。

In an infectivity assay, human illeocecal adenocarcinoma (HCT-8) cellswere infected with different concentrations of Cryptosporidium parvum oocysts. After 48 hours of incubation, in-situ hybridization (ISH) using digoxigenin-labeled DNA probes targeted at the C. parvum-specific small subunit rRNA sequences with colorimetric detection was performed to detect infectious oocysts. In addition, immunofluorescencedetection of intracellular reproductive stages of C. parvum using fluorescein-labeled polyclonal antibody against sporozoites of C. parvum was conducted and compared with ISH. Both C. parvumand C. muris were used to infect HCT-8 cells along with ISH to determine the specificity of the DNA probes. Includes 18 references, tables, figures.