大肠杆菌O157:H7是出血性结肠炎、非血性腹泻和溶血性尿毒症综合征的病原体。大肠杆菌O157:H7是一种重要的水传细菌病原体。最近的疫情集中关注饮用水在这种微生物传播中的作用。研究表明，该病原体对氯化反应敏感，对大肠杆菌属菌株的耐药性相似，但在未经处理的饮用水系统中，它仍然是一个问题。与任何微生物病原体一样，快速检测病原体存在的能力可能会大大增强公共卫生当局应对疫情的能力。本研究旨在评估一种快速检测大肠杆菌的方法。 大肠杆菌O157:H7在水中。使用流式细胞仪直接从水样中检测有机物的存在，并在预富集和免疫磁分离后进行检测。本研究中使用了亲和纯化的荧光标记的大肠杆菌O157:H7特异性多克隆抗体。包括表格，如图所示。

Escherichia coli O157:H7 serves as the etiological agent for hemorrhagic colitis,non-bloody diarrhea, and the hemolytic uremic syndrome. E. coli O157:H7 hasemerged as an important waterborne bacterial pathogen. Recent outbreaks havecentered attention on the role of drinking water in the transmission of thisorganism. It has been shown that the pathogen is sensitive to chlorination and issimilar in resistance to generic strains of E. coli, however, it continues to bea problem in untreated drinking water systems. As with any microbial pathogen,the ability to rapidly detect the presence of the pathogen may greatly enhancethe ability of public health authorities to respond in an outbreak. This studywas designed to evaluate a rapid method for the detection of E. coli O157:H7 inwater. A flow cytometric instrument was used to detect the presence of theorganism directly from water samples and after pre-enrichment and immunomagneticseparation. Affinity purified fluorescently labeled polyclonal anitbodiesspecific for E. coli O157:H7 were used in the study. Includes tables, figure.