1.1 本测试方法旨在评估用于传统顶部装载自动洗衣操作的消毒/消毒洗衣粉/添加剂。该测试方法主要旨在提供具有代表性的营养细菌测试，但也可以设计为适应真菌和病毒的测试。 注1: 本测试方法不评估用于前装或顶装、低水高效自动洗衣操作的消毒/消毒剂洗衣粉/添加剂。 1.2 本试验方法旨在补充试验方法 E2406 并且将用于该测试方法被确定为产品使用的最坏情况的情况。 1.3 这些程序需要微生物技术知识。 1.4 研究者有责任确定是否需要良好实验室规范（GLP），并在适当的情况下遵守这些规范（见《美国联邦法规》第40章第160部分或修订版） 1.5 以国际单位制表示的值应被视为标准值。本标准不包括其他计量单位。 注2: 在本试验方法中，所有应用均使用公制单位，但距离除外，在这种情况下使用英寸。 1.6 当本文未指定测试生物体时，可能需要对测试方法进行适当的修改。 1.7 本标准并不旨在解决与其使用相关的所有安全问题（如果有的话）。本标准的使用者有责任在使用前建立适当的安全、健康和环境实践，并确定监管限制的适用性。 1.8 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 =====意义和用途====== 5.1 本试验方法中的程序用于评估试验试剂（抗菌剂/活性成分）或制剂在单次洗涤后减少或完全杀死织物和洗涤水中细菌种群的活性。常见顶部装载机中的水与织物比是动态的，因地区而异。在测试之前，应确定最坏情况下的适当水与织物比和温度。如果确定最坏的情况发生在顶部装载高效洗衣机中，则可能需要修改此测试方法。

1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in traditional top-loading automatic clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses. Note 1: This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading or top-loading, low water high efficiency automatic clothes washing operations. 1.2 This test method is intended to compliment Test Method E2406 and is to be used in the cases where this test method is determined to be the worse case scenario for product usage. 1.3 Knowledge of microbiological techniques is required for these procedures. 1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, Part 160 or as revised.) 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. Note 2: In this test method, metric units are used for all applications, except for distance in which case inches are used. 1.6 Appropriate modifications to the test method may be required when the testing organisms are not specified herein. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 The procedure in this test method is used to evaluate the activity of a test reagent (antimicrobial agent/active ingredient) or formulation in the reduction or complete kill of the bacterial population in fabric and wash water following a single wash. The water to fabric ratio in common top loading machines is dynamic and varies by region. The proper water to fabric ratio and temperature for the worse-case scenario should be determined prior to testing. This test method may need to modified if the worse-case scenario is determined to be in top loading high efficiency washing machine.