1.1 本标准介绍了测定生物酸化对混凝土产品的影响和/或抗菌产品抵抗混凝土微生物诱导腐蚀（MIC）的功效的试验方法。在这些试验中，生物酸化是通过硫氧化细菌（SOB）实现的，该细菌可以在不使用H的情况下将元素硫或硫代硫酸盐转化为硫酸 2. S气体。 1.2 本标准在混凝土产品MIC指南文件中引用。指导 1894元 提供了有关混凝土产品微生物诱导腐蚀的指南，并概述了可以和应该在何处使用此测试及其选项。 本文件并非混凝土产品MIC的指导文件。 1.3 本标准不包括受控繁殖室试验，其中H 2. S气体是由细菌活动产生的，酸化是该H转化的结果 2. S气体转化为硫酸。 1.4 本标准不包括化学酸浸试验，其中酸化是通过添加化学硫酸而不是通过细菌活性实现的。《试验方法》中描述了化学酸浸试验方案 C267号 和 1898年 . 1.5 本标准不包括评估现场暴露条件或污水管、混凝土罐或混凝土立管网络设计的试验。 1.6 本标准不包括在下水道中监测混凝土试件或其他试样的现场试验。 1.7 本标准中所述的试验不应在已经暴露于MIC条件下的混凝土样品上进行。 1.8 本标准不包括由于化学硫酸盐侵蚀导致的混凝土劣化，化学硫酸盐侵蚀是由废水中存在的硫酸盐化合物与水泥水化产物反应引起的。《试验方法》提供了评估硫酸盐侵蚀的试验方法 C452号机组 和 1012/1012米 . 1.9 以国际单位表示的数值视为标准值。 本标准不包括其他计量单位。 1.10 本标准并不旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.11 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 =====意义和用途====== 4.1 如指南中所述 1894元 混凝土的MIC被认为是一个三阶段过程，pH值降低（第一阶段）（例如，12.5>pH>9-10），生物膜的建立进一步降低pH值（第二阶段）（如，9-10>pH>4-6），最终由于生物酸暴露而恶化（第三阶段）（比如，<~4 pH）。本标准提供了标准试验方法，以评估MIC不同阶段对混凝土产品的影响以及混凝土中使用的抗菌产品的功效。 4. 2. 测试是在实验室中生长的含有受控细菌菌株的模拟暴露溶液中进行的。这些试验不需要环境室，拟在生物安全1级实验室条件下作为台式试验进行。这些测试适用于模拟MIC的第二阶段和第三阶段，因为溶液的pH范围可以控制在每个阶段的范围内。 4.3 本标准提供了三种试验方法。 4.3.1 试验方法A适用于评估抗菌添加剂在延缓或防止营养素中生物酸化方面的功效- 富模拟废水暴露溶液。 4.3.2 试验方法B适用于评估规定水泥体系（方案B1）中抗菌外加剂的有效性，或评估不同水泥体系（选项B2）在MIC第二阶段延迟或防止混凝土微生物腐蚀方面的性能。 4.3.3 试验方法C适用于评估胶结系统在MIC第三阶段延迟或防止混凝土微生物腐蚀方面的适用性。 4.4 通过这些试验方法获得的结果应作为指南中使用的信息 1894元 在特定应用中选择生物抗性材料，但不是唯一的依据。未尝试将实际使用时可能影响材料性能的所有各种因素纳入这些试验方法。

1.1 This standard presents test methods for the determination of the effects of biogenic acidification on concrete products and/or efficacy of antimicrobial products to resist microbially-induced corrosion (MIC) of concrete. In these tests, the biogenic acidification is achieved by sulfur-oxidizing bacteria (SOB) that can convert elemental sulfur or thiosulfate to sulfuric acid without the use of H 2 S gas. 1.2 This standard is referenced in the guideline document for MIC of concrete products. Guide C1894 provides guidance for microbially-induced corrosion of concrete products and an overview of where this test, and its options, can and should be used. This document is not intended to be a guideline document for MIC of concrete products. 1.3 This standard does not cover controlled breeding chamber tests, in which H 2 S gas is produced by bacterial activity and acidification is the result of the conversion of this H 2 S gas to sulfuric acid. 1.4 This standard does not cover chemical acid immersion tests, in which acidification is achieved by chemical sulfuric acid addition, not by bacterial activity. Testing protocols for chemical acid immersion are described in Test Methods C267 and C1898 . 1.5 This standard does not cover tests that assess field exposure conditions or sewage pipe, concrete tank, or concrete riser network design. 1.6 This standard does not cover live trial tests where concrete coupons or other specimens are monitored in sewers. 1.7 The tests described in this standard should not be performed on concrete samples that have already been exposed to MIC conditions. 1.8 This standard does not cover concrete deterioration due to chemical sulfate attack, which is caused by the reaction of sulfate compounds that exist in wastewater with the hydration products of cement. Test methods for assessing sulfate attack are provided by Test Methods C452 and C1012/C1012M . 1.9 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.10 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.11 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 As described in Guide C1894 , the MIC of concrete is considered to be a three-stage process with the reduction in pH (Stage I) (for example, 12.5 > pH > 9-10), the establishment of biofilms which further lowers the pH (Stage II) (for example, 9-10 > pH > 4-6) and eventual deterioration due to biogenic acid exposure (Stage III) (for example, < ~4 pH). This standard provides standard test methods to assess the effects of different stages of MIC on concrete products and efficacy of antimicrobial products used in or on concrete. 4.2 The tests are performed in simulated exposure solutions containing well-controlled bacterial strains that are grown in the laboratory. These tests do not require an environmental chamber and are intended to be performed as benchtop tests in biosafety level 1 laboratory conditions. These tests are suitable for simulation of the Stage II and III of MIC because the pH range of the solution can be controlled within the ranges of each stage. 4.3 This standard provides three test methods. 4.3.1 Test Method A is suitable for assessing the efficacy of antimicrobial admixtures in delaying or preventing biogenic acidification in a nutrient-rich simulated wastewater exposure solution. 4.3.2 Test Method B is suitable for assessing the effectiveness of antimicrobial admixtures in a prescribed cementitious system (Option B1) or assessing the performance of different cementitious systems (Option B2) in delaying or preventing microbially-induced corrosion of concrete in the Stage II of MIC. 4.3.3 Test Method C is suitable for assessing the suitability of cementitious systems in delaying or preventing microbially-induced corrosion of concrete in the Stage III of MIC. 4.4 The results obtained by these test methods should serve as information to be used with Guide C1894 in, but not as the sole basis for, selection of a biologically-resistant material for a particular application. No attempt has been made to incorporate into these test methods all the various factors that may affect the performance of a material when subjected to actual service.