1.1 本试验方法用于评估消毒剂对预清洁、无生命、坚硬、无孔、无食品接触表面的抗菌效果 金黄色葡萄球菌 或 肺炎克雷伯菌 或 产气克雷伯菌 或其组合。当测试本文未规定的生物体时，可能需要对方法进行适当修改。当使用本文未描述的试验表面时（见试验方法 第2274页 )或者当评估基于喷雾或毛巾的抗菌产品时，也可能需要修改。 1.2 该试验方法也可用于评估一种抗生素的抗菌效果- 建议用于轻度污染、无生命、无孔、非食品接触表面的清洁剂。 1.3 研究人员有责任确定是否需要良好实验室规范（GLP），并在适当的情况下遵循这些规范（见《美国联邦法规》第40卷第160节或修订版） 1.4 以SI单位表示的值应视为标准值。本标准不包括其他计量单位。 1.5 本标准可能涉及危险材料、化学品和微生物，只能由受过正式微生物培训的人员执行。 本标准并不旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前建立适当的安全、健康和环境实践，并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 =====意义和用途====== 4.1 本试验方法应用于确定拟用作非食品接触消毒剂或一步清洁消毒剂的化学品与对照相比，是否能减少处理载体上选定试验微生物的百分比。

1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned, inanimate, hard, nonporous, non-food contact surfaces against Staphylococcus aureus , or Klebsiella pneumoniae or Klebsiella aerogenes , or a combination thereof. Appropriate modifications to the method may be required when testing organisms not specified herein. When utilizing test surfaces not described herein (see Test Method E2274 ) or when evaluating spray-based or towelette-based antimicrobial products, modifications may also be required. 1.2 This test method may also be used to evaluate the antimicrobial efficacy of one-step cleaner-sanitizer formulations recommended for use on lightly soiled, inanimate, nonporous, non-food contact surfaces. 1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.) 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard may involve hazardous materials, chemicals and microorganisms and should be performed only by persons who have had formal microbiological training. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 This test method shall be used to determine if a chemical intended for use as a non-food contact sanitizer or as a one-step cleaner-sanitizer provides percent reductions of the selected test organisms on treated carriers as compared to control.