美国地质调查局最近的一项研究表明，亚利桑那州的地表水 广泛污染内分泌干扰物（EDC），包括各种雌激素 化合物。人们对饮用水处理过程中EDC的命运知之甚少。大部分 全国各地的水处理厂都使用氯作为消毒剂。HOCl能够 氧化含胺官能团和/或环氮的有机化合物是很好的选择 建立了。许多EDC/PPCP化合物含有苯环，这会影响其性能 与氯和转化产物的反应性也可表现出不同程度的雌激素活性。 鉴于这些化学物质存在于微量元素中，这个问题变得尤为重要 浓度水平。本研究的目的是描述在动物中发现的雌激素的类型 水源及其对先进水处理工艺的响应，这有助于 了解饮用水中EDC的去除和转化。 来自佛得河、盐河和亚利桑那州中部项目的水样 （CAP）运河和两个当地的水处理厂被收集在一个琥珀色的玻璃瓶中 每月一次。浓缩前，将水样分为两组。一套样品 另一组未经处理。这两组都是液-液浓缩的 用二氯甲烷萃取。分析浓缩样品中是否存在 使用在12孔培养皿中生长的MCF细胞系进行内分泌干扰物（雌激素）。这个 17b雌二醇作为所有细胞增殖试验的标准。 计算了源水和成品水的相对增殖效应（RPE）。这个 盐河、佛得河、CAP canal、水处理原水中的雌激素 1号厂（WTP1）和2号水处理厂（WTP2）的平均值分别为62.46%、66.69%和63.06%， 35.87%和29.87%。这些水平表明这些药物中存在部分到完全激动性的化学物质 水源。WTP2和WTP1成品水的相对增殖效应平均 24.31%和23.58%，表明治疗过程能够降低 水处理过程中的雌激素化学物质。相对增殖效应 所有来源的含氯原水被发现总是低于各自的未含氯原水 原水，但大于成品饮用水。 这种方法表明，它有望 全面评估高级治疗流程的有效性，以缓解 饮用水中的累积雌激素。包括数字。

A recent U.S. Geological Survey study has demonstrated that surface waters in the state of Arizona have widespread contamination of endocrine disrupting compounds (EDCs) and including various estrogenous compounds. Little is known about the fate of EDCs in drinking water treatment processes. Most of the water treatment plants around the nation use chlorine as a disinfectant. The ability of HOCl to oxidize organic compounds with amine functional groups and/or cyclic nitrogen is well established. Many of the EDC/PPCP compounds contain benzene rings, which impact their reactivity with chlorine and the transformed product can also show varying degree of estrogenicity. The issue becomes especially important in light of the fact that these chemicals occur at trace concentration levels. The objective of this study was to characterize the type of estrogenicity found in source water and its response to advanced water treatment processes, which can help in understanding the removal and transformation of EDCs from drinking water. The water samples from the Verde River, the Salt River, the Central Arizona Project (CAP) canal, and two local water treatment plants were collected in amber glass bottles on a monthly basis. The water samples were split into two sets before concentration. One set of samples was chlorinated and the other set was kept untreated. Both the sets were concentrated by liquid-liquid extraction using Dichloromethane. The concentrated samples were assayed for the presence of Endocrine Disrupting Chemicals (estrogenic) by using MCF cell line grown in 12 well plates. The 17b-estradiol was used as standard in all cell proliferation assays. The Relative Proliferation Effect (RPE) for source and finished waters were calculated. The estrogenicity in water from the Salt River, the Verde River, CAP canal, raw water from water treatment plant 1 (WTP1) and water treatment plant 2 (WTP2) averaged at 62.46%, 66.69%, 63.06%, 35.87%, and 29.87%. These levels suggest the presence of partial to fully agonistic chemicals in these source waters. The relative proliferation effect for finished water from WTP2 and WTP1 averaged at 24.31% and 23.58%, suggesting that treatment processes were able to reduce the levels of estrogenic chemicals during water treatment processes. The relative proliferation effect for chlorinated raw waters from all sources were found to be always less than respective unchlorinated raw waters but greater than finished drinking water. This approach shows a promise to comprehensively evaluate the effectiveness of advanced treatment processes to mitigate cumulative estrogenicity in drinking water. Includes figures.