1.1 本实践旨在评估水混溶性金属加工液的相对固有生物抗性、可归因于抗菌农药增强的生物抗性或两者兼而有之。它取代了测试方法 D3946 和 E686 . 1.2 在该实践中，通过用生物接种物挑战金属加工流体来确定相对生物抗性，所述生物接种物可以是表征的(由一种或多种已知的生物培养物组成)或未表征的(由生物污染的金属加工流体或来自变质金属加工流体的一种或多种未鉴定的分离物组成)。挑战流体生物抗性定义为对生物量增加、活细胞回收率增加、化学性质变化、物理性质变化或其某种组合的抗性。1.3 本实践适用于掺入金属加工液浓缩液或最终使用稀释液中的抗微生物剂。它还适用于使用非杀微生物的固有生物抗性组分配制的金属加工液。 1.4 以SI单位表示的值将被视为标准值。本标准不包括其他计量单位。 1.5 本标准并不旨在解决与其使用相关的所有安全性问题（如果有）。本标准的使用者有责任在使用前建立适当的安全、健康和环境实践并确定法规限制的适用性。 1.6 本国际标准是根据世界贸易组织发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的o贸易委员会。 ======意义和用途====== 5.1 本实施规程提供了评价金属加工液配方的相对生物抗性、确定在金属加工系统中使用流体之前或期间是否需要添加杀微生物剂以及评估杀微生物剂性能的实验室程序。提供了在实践中选择杀微生物剂的一般考虑 E2169 . 5.2 影响再循环金属加工液系统中攻击种群数量、分类多样性、生理状态、接种频率和生物降解效应的因素多种多样，并且仅部分了解。因此，根据本规范完成的试验结果只能用于比较试验系列中产品或杀微生物剂处理的相对性能。结果不应被解释为预测实际现场性能。

1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Test Methods D3946 and E686 . 1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change, physical property change or some combination thereof. 1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant components. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This practice provides laboratory procedures for rating the relative bioresistance of metalworking fluid formulations, for determining the need for microbicide addition prior to or during fluid use in metalworking systems and for evaluating microbicide performance. General considerations for microbicide selection are provided in Practice E2169 . 5.2 The factors affecting challenge population numbers, taxonomic diversity, physiological state, inoculation frequency and biodeterioration effects in recirculating metalworking fluid systems are varied and only partially understood. Consequently, the results of tests completed in accordance with this practice should be used only to compare the relative performance of products or microbicide treatments included in a test series. Results should not be construed as predicting actual field performance.