1.1 该测试方法旨在评估接触介导的微生物在二维材料表面的转移。可使用本试验方法评估旨在减少微生物粘附或污染的材料表面。此外，该试验方法可用于其他下游工艺的各种材料表面。 1.2 该测试方法旨在通过触摸转移量化细菌在材料表面的转移，成本低廉，需要有限的实践培训，并且适用于许多表面或微生物。非多孔表面被接种的滤纸作为载体污染，表面污染通过使用复制生物体检测和计数（RODAC）板采样进行量化。 1.3 执行本试验方法需要基本微生物学培训。 1.4 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 4.1 该测试方法将滤纸用作评估细菌接触材料表面的介质。将样品表面暴露于浸透水的滤纸中 金黄色葡萄球菌 悬浮，然后用复制生物体检测和计数（RODAC）板从表面回收细菌。 此测试方法报告日志 10 与对照表面相比，预期试验表面上的细菌转移减少。只要材料相同，测试表面和控制表面可以因纹理、涂层、处理或任何其他所需变量而不同。

1.1 This test method is designed to evaluate the contact-mediated microbial transference on two-dimensional material surfaces. Material surfaces intended to reduce microorganism adherence or contamination may be evaluated using this test method. Additionally, this test method can be utilized on a wide variety of material surfaces for other downstream processes. 1.2 This test method is designed to quantify the fomite transfer of bacteria on material surfaces through touch-transfer, be inexpensive, require limited training in practice, and be adaptable to many surfaces or microorganisms. Non-porous surfaces are contaminated with inoculated filter paper as a carrier and surface contamination is quantified by sampling with Replicate Organism Detection and Counting (RODAC) plates. 1.3 Basic microbiology training is required to perform this test method. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 This test method utilizes filter paper as a medium for evaluating touch-transfer of bacteria to material surfaces. Sample surfaces are exposed to filter papers saturated with a Staphylococcus aureus suspension, followed by recovery of the bacteria from the surface with Replicate Organism Detection and Counting (RODAC) plates. This test method reports the log 10 reduction of bacterial transfer on an intended test surface compared to a control surface. The test and control surfaces can differ by texture, coating, treatment, or any other desired variables, as long as they are the same material.