ISO 15366-2:2014描述了在通过质谱法或阿尔法光谱法等进行同位素分析之前，对辐照轻水反应堆燃料溶解溶液和乏燃料后处理厂高活性废液样品中的铀和钚进行化学分离和提纯的程序。ISO 15366-2:2014描述了一种与ISO 15366-1略有不同的分离技术，基于相同的化学成分，使用更小的柱、不同的支撑材料和特殊的纯化步骤，适用于含有纳克及以下钚和铀量的样品。 检测限为500 pg钚和500 pg铀。

ISO 15366-2:2014 describes procedures to chemically separate and purify uranium and plutonium in dissolved solutions of irradiated light water reactor fuels and in samples of high active liquid waste of spent fuel reprocessing plants, prior to their isotopic analysis by e.g. mass spectrometric method or alpha spectrometry. ISO 15366-2:2014 describes a slightly different separation technique from ISO 15366-1, based on the same chemistry, using smaller columns, different support material and special purification steps, applicable to samples containing plutonium and uranium amounts in the nanogram range and below. The detection limits were found to be 500 pg plutonium and 500 pg uranium.