1范围 ISO 11268的本部分规定了评估土壤生境功能和通过皮肤和消化吸收测定土壤污染物和化学品对赤子爱胜蚓/安德烈爱胜蚓的急性毒性的方法之一。适用于未知质量的土壤和土壤材料，例如来自污染场地、改良土壤、修复后的土壤、农业或其他相关场地的土壤和废物。 使用标准土壤（最好是规定的人工土壤基质）评估物质的影响。对于污染土壤，在试验土壤和对照土壤中确定对存活的影响。根据本研究的目的，控制和稀释基质（污染土壤的稀释系列）应为与待测试土壤样品（参考土壤）相当的未污染土壤，或标准土壤（例如人工土壤）。 提供了如何在温带和热带条件下使用该方法测试化学品的信息。 该方法不适用于挥发性物质，即H（亨利常数）或空气/水分配系数大于1的物质，或25°C时蒸汽压力超过0013 Pa的物质。 该方法未考虑试验期间物质或污染物的可能降解。

1 Scope This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects on survival are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil). Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions. The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C. This method does not take into account the possible degradation of the substances or contaminants during the test.