Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp.
食物链的微生物学 - 沙门氏菌检测 计数和血清分型的水平方法 - 第1部分:沙门氏菌的检测
发布日期:
2017-02-23
ISO 6579-1:2017规定了检测沙门氏菌的水平方法。适用于以下情况:
-供人类食用和动物饲养的产品;
-食品生产和食品处理领域的环境样本;
-初级生产阶段的样本,如动物粪便、灰尘和拭子。
通过这种水平方法,大多数沙门氏菌血清型都将被检测出来。为了检测某些特定血清型,可能需要额外的培养步骤。对于伤寒沙门氏菌和副伤寒沙门氏菌,程序见附录D。
选择性富集培养基改良半固体Rappaport Vassiliadis(MSRV)琼脂用于检测运动型沙门氏菌,不适用于检测非运动型沙门氏菌菌株。
ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling;
- samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.