本文件涉及人类因摄入土壤和土壤材料而暴露的评估。它规定了一种基于生理学的测试程序，用于评估与人体口服吸收有关的暴露，从而评估污染土壤中金属的人体生物可接受性。 该方法是使用合成胃肠液进行的连续提取，可用于评估口服生物利用度。在模拟人体胃肠道基本物理化学条件的环境中，以筛分形式提取土壤或其他地质材料。 本文件描述了一种模拟通过人体胃肠道三个部分（口、胃和小肠）后土壤和土壤材料中金属释放的方法。 它产生的提取物代表人体胃肠道中潜在有害元素的浓度，用于后续的化学表征。 注1:生物利用度可用于近似口服生物利用度。 注2:本试验已在实验室间试验中验证砷、镉和铅。该方法已通过体内验证，以评估砷、镉和铅的口服生物利用度。

This document deals with the assessment of human exposure from ingestion of soil and soil materials. It specifies a physiologically based test procedure for the estimation of the human bioaccessibility of metals from contaminated soil in connection with the evaluation of the exposure related to human oral uptake. The method is a sequential extraction using synthetic gastrointestinal fluids and can be used to estimate oral bioaccessibility. Soils or other geological materials, in sieved form, are extracted in an environment that simulates the basic physicochemical conditions of the human gastrointestinal tract. This document describes a method to simulate the release of metals from soil and soil materials after passage through three compartments of the human gastrointestinal tract (mouth, stomach and small intestine). It produces extracts that are representative of the concentration of potentially harmful elements in the human gastrointestinal tract for subsequent chemical characterization. NOTE 1 Bioaccessibility can be used to approximate oral bioavailability. NOTE 2 The test has been validated for arsenic, cadmium and lead in an interlaboratory trial. The method has been in vivo validated to assess the oral bioavailability of arsenic, cadmium and lead.