1.1 本试验方法包括一项分析试验，用于测定与天然橡胶、乳胶和弹性体产品相关的水溶性可提取蛋白质总量。水溶性蛋白质在缓冲溶液中提取，然后沉淀浓缩，并将其与可能干扰测定的水溶性物质分离。使用蛋白质标准，通过改良的Lowry方法重新溶解提取的蛋白质并进行比色定量。 1.2 就本试验方法而言，蛋白质测量范围将基于检测和定量极限，并以微克/分为单位记录 2. 试样。 1.3 该测试方法设计为准确且与工业环境兼容。 1.4 本试验方法包括将干扰物质的影响降至最低的步骤。 1.5 众所周知，存在其他可沥滤蛋白质分析方法，这些方法可用于常规质量控制目的，前提是这些方法已经过验证，并与本试验方法规定的参考方法建立了相关性。 1.6 该测试方法尚未验证是否适用于避孕套等润滑产品。通常市面上销售的含有不同润滑剂的避孕套尚未在ASTM ILS中进行测试，以确定润滑剂是否以及在何种程度上干扰了检测。 1.7 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.8 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5. 1. 本试验方法用于测定乳胶中的蛋白质含量，主要用于测试天然橡胶、乳胶和弹性材料的残余蛋白质含量。假设所有使用该测试方法的人都是经过培训的分析员，能够熟练、安全地执行常见的实验室程序。预计工作将在配备适当的实验室中进行。

1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. 1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm 2 test specimen. 1.3 The test method is designed to be accurate and compatible with the industrial environment. 1.4 Steps are included in this test method to minimize the effects of interfering substances. 1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method. 1.6 This test method has not been validated for use with lubricated products such as condoms. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so to what degree, the lubricant interferes with the assay. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This test method, for the determination of protein levels in latex, is primarily intended to test NR, latex, and elastomeric materials for residual protein content. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely. It is expected that work will be performed in a properly equipped laboratory.