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现行 ASTM E685-93(2021)
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Standard Practice for Testing Fixed-Wavelength Photometric Detectors Used in Liquid Chromatography 用于液相色谱的固定波长光度检测器的标准实践
发布日期: 2021-04-01
1.1 本规程旨在作为测试光度检测器(PD)性能的指南,该光度检测器(PD)用作在210范围内的一个或多个固定波长下运行的液相色谱(LC)系统的检测组件 纳米至800纳米。如果可能,可在254 nm处进行测量,并可在其他波长处进行选择。 1.2 本规程旨在描述检测器的性能,该性能独立于色谱系统(静态条件)和流动溶剂(动态条件)。 1.3 有关一般液相色谱程序,请参阅参考文献 ( 1- 9 ). 2. 1.4 有关液相色谱检测器的原理、结构、操作和评估的一般信息,请参阅参考文献 ( 10和 11 ) 除了参考文献中专门介绍探测器的章节之外 ( 1- 7. ). 1.5 本标准并非旨在解决与其使用相关的所有安全问题(如有)。本标准的用户有责任在使用前制定适当的安全、健康和环境实践,并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒(TBT)委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 4.1 虽然可以在不同和独特的条件下观察和测量探测器的每个特性,但本实践的目的是获得一套完整的探测器规范 在相同的操作条件下。 还应注意的是,为了完全指定探测器的能力,应在探测器有效范围内的几组条件下测量其性能。本实施规程中描述的术语和测试具有足够的通用性,因此无论最终操作参数如何,都可以使用这些术语和测试。 4.2 记录器或其他读出装置的线性度和响应时间应确保不会扭曲或干扰检测器的性能。这需要根据制造商的指示调整增益、阻尼和校准。如果在探测器和最终读出装置之间使用额外的电子滤波器或放大器,也应首先确定其特性。
1.1 This practice is intended to serve as a guide for the testing of the performance of a photometric detector (PD) used as the detection component of a liquid-chromatographic (LC) system operating at one or more fixed wavelengths in the range 210 nm to 800 nm. Measurements are made at 254 nm, if possible, and are optional at other wavelengths. 1.2 This practice is intended to describe the performance of the detector both independently of the chromatographic system (static conditions) and with flowing solvent (dynamic conditions). 1.3 For general liquid chromatographic procedures, consult Refs ( 1- 9 ). 2 1.4 For general information concerning the principles, construction, operation, and evaluation of liquid-chromatography detectors, see Refs ( 10 and 11 ) in addition to the sections devoted to detectors in Refs ( 1- 7 ). 1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 Although it is possible to observe and measure each of the several characteristics of a detector under different and unique conditions, it is the intent of this practice that a complete set of detector specifications should be obtained under the same operating conditions. It should also be noted that to completely specify a detector’s capability, its performance should be measured at several sets of conditions within the useful range of the detector. The terms and tests described in this practice are sufficiently general that they may be used regardless of the ultimate operating parameters. 4.2 Linearity and response time of the recorder or other readout device used should be such that they do not distort or otherwise interfere with the performance of the detector. This requires adjusting the gain, damping, and calibration in accordance with the manufacturer's directions. If additional electronic filters or amplifiers are used between the detector and the final readout device, their characteristics should also first be established.
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归口单位: E13.19
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