Biotechnology — Cell counting — Part 1: General guidance on cell counting methods
生物技术 - 细胞计数 - 第1部分:细胞计数方法的一般指导
发布日期:
2018-01-19
ISO 20391-1:2018定义了与生物技术细胞计数相关的术语。它描述了悬浮细胞(通常为细胞浓度)和粘附在基质上的细胞(通常为细胞的面积密度)的计数。它为一般计数方法(包括总计数和差分计数、直接计数和间接计数)以及方法选择、测量过程、数据分析和报告提供了关键考虑因素。
ISO 20391-1:2018适用于所有细胞类型的计数?哺乳动物和非哺乳动物(如细菌、酵母)细胞。
ISO 20391-1:2018不适用于组织切片或生物材料基质中的细胞计数。
目前存在多个针对特定行业/应用的细胞计数国际和国家标准。适用时,用户可在其范围内(特定测量技术和/或应用)操作时参考现有标准。
ISO 20391-1:2018 defines terms related to cell counting for biotechnology. It describes counting of cells in suspension (generally cell concentration) and cells adhered to a substrate (generally area density of cells). It provides key considerations for general counting methods (including total and differential counting, and direct and indirect counting) as well as for method selection, measurement process, and data analysis and reporting.
ISO 20391-1:2018 is applicable to the counting of all cell types?mammalian and non-mammalian (e.g. bacteria, yeast) cells.
ISO 20391-1:2018 is not intended for counting of cells while in a tissue section or a biomaterial matrix.
Several sector/application-specific international and national standards for cell counting currently exist. When applicable, the user can consult existing standards when operating within their scope (specific measurement techniques and/or applications).