Infant formula and adult nutritionals. Determination of <i>trans</i> and total (<i>cis</i> + <i>trans</i>) vitamin K<sub>1</sub> content. Normal phase HPLC
婴儿配方奶粉和成人营养品 测定<i>反式</i>和总(<i>顺式</i>+<i>反式</i>)维生素K<sub>1</sub>含量 正相高效液相色谱法
发布日期:
2019-01-09
BS ISO 21446:2019规定了使用柱后还原和荧光检测的正相(NP)高效液相色谱法(HPLC)定量测定婴儿、儿童和成人营养品中反式和总(顺式+反式)维生素K1的方法。该方法在~2µg/l至80µg/l反式维生素K1的标准范围内表现出良好的线性,假设将4克样品稀释至10毫升,则标准品的定量限(LOQ)估计为0,4µg/l,样品的定量限(RTF)估计为0,09µg/100 g。
交叉引用:ISO 5725-2:1994OMA 2015.09EN 14148购买本文件时可获得的所有现行修改件均包含在购买本文件中。
BS ISO 21446:2019 specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 µg/l to 80 µg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 µg/l for standards and 0,09 µg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.Cross References:ISO 5725-2:1994OMA 2015.09EN 14148All current amendments available at time of purchase are included with the purchase of this document.