使用准直光束装置在实验室规模上研究了中压紫外线（UV）对成品饮用水中微小隐孢子虫卵囊的灭活作用，并使用Calgon Carbon Corporation Sentinel（TM）系统在示范规模上进行了研究。通过体外（DAPI/PI和最大化体外脱囊）和体内（新生小鼠感染性）试验评估卵囊活力。使用新生小鼠传染性试验，实验室规模的研究表明，紫外线剂量低至41 mJ cm-2时，灭活率>4对数；体外替代试验显示，在这种和更高的紫外线剂量下，很少或没有失活。体外试验表明，卵囊的活力大大高估了在易感宿主中防止卵囊感染所需的紫外线剂量。在NSF/EPA ETV项目下进行的示范研究提供了与试验台一致的结果- 结果表明，低至19 mJ cm-2的紫外线剂量可使隐孢子虫卵囊失活3.9对数。

Inactivation of Cryptosporidium parvum oocysts in finished drinking water by medium-pressure ultraviolet (UV) light was investigated at bench-scale using a collimated beam apparatus, and at demonstration scale using the Calgon Carbon Corporation Sentinel(TM) system. Oocyst viability was assessed using in vitro (DAPI/PI and maximized in vitro excystation) and in vivo (neonatal mouse infectivity) assays. Using the neonatal mouse infectivity assay, the bench-scale studies showed >4 log inactivation at UV doses as low as 41 mJ cm-2; the in vitro surrogate assays showed little or no inactivation at this and higher UV doses. The in vitro assays, which indicate oocyst viability, grossly overestimated the UV doses required to prevent infection by the oocysts in susceptible hosts. The demonstration studies, carried out under the NSF/EPA ETV program, provided results that agreed with the bench-scale results and showed that a UV dose as low as 19 mJ cm-2 provided 3.9 log inactivation of Cryptosporidium oocysts.