This article describes research conducted at the Colorado State University Engineering Research Center in Fort Collins, Colorado, to ascertain the suitability of diatomaceous earth (DE) for the removal of Giardia cysts. Removals of turbidity, total coliform bacteria, standard plate count bacteria, and particles were also determined. The following operating conditions were examined: DE grade, hydraulic loading rate, influent concentrations of bacteria and Giardia cysts, head loss, run time, temperature, duration of filtration run, and the use of alum-coated DE. Pilot plant tests indicate that DE filtration is virtually 100 percent effective in Giardia cyst removal for all DE grades over a wide range of conditions. Grade is an important factor in the removal of bacterial and turbidity. Alum coated DE significantly improved removals of turbidity and bacteria. Includes 23 references, tables, figures.